ABSTRACT
Mutation breeding is a powerful and effective tool to upgrade well-adapted varieties by altering one or few major agronomic traits which limit their productivity and quality. Improving the yield and fruit quality traits of Katugastota Wilt Resistant (KWR), a tomato variety tolerant to bacterial wilt disease was aimed owing to the low success in developing of bacterial wilt tolerance through conventional breeding. Present study was conducted to determine the effective dosage of gamma irradiation for KWR seeds that creates maximum genetic variation by evaluating seedling traits in M1 population. KWR seeds were gamma irradiated with four doses (100, 200, 300, 400 Gy) and compared with untreated control in an experimental set up using Complete Randomized Design with 3 replicates. The results revealed that gamma doses had different responses on morphological traits in M1 seedlings. Among the traits considered, days taken for germination, seedling height, root length and number of roots were significantly affected by the gamma doses. Effective doses which were determined based on seedling height and root length were 398.75 Gy and 371.69 Gy, respectively. Based on the effective doses, gamma radiation dosage between 370 Gy to 400 Gy could be identified as the dosage that creates maximum genetic variation in variety KWR. Key Words: Effective dosage, Gamma rays, Irradiation, Katugstota Wilt Resistant, Tomato
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